VOGEL LAB

Interdisciplinary investigation of cellular division machinery
McGill University

ABOUT US

We use budding yeast to investigate the assembly and function of the mitotic spindle, and the control systems that govern asymmetric cell division.

Biological

Understanding spindle assembly through mutant analysis, high throughput genetic screening, protein-protein interaction assays, experimental evolution.

Computational

Applying modern computational methods to process and model complex biological data for hypothesis generation.

Physical

Elucidating the dynamics and forces that govern microtubule spindle assembly and function combining physical models and in-vivo fluorescence microscopy.

RECENT PUBLICATIONS

Dynamic Properties of Protein Complexes

Microtubule Pairing in Spindle Assembly

PEOPLE

Jackie Vogel

JACKIE VOGEL

Group Leader

Jackie Vogel trained as a biochemist, focusing on mechanisms of centrosome maturation (Ph.D. University of Kansas, Ida Hyde and Candlin Scholar). Jackie studied the role of gamma-tubulin regulation in cell division at Yale University as a NIH and Leslie Warner post doctoral fellow (1996 - 2001). At McGill, Jackie has been a CIHR New Investigator (2004-2009) and most recently held the McGill-Canadian Pacific Biotechnology research chair (2009-2014). She is currently leading the Quantitative Biology Initiative at McGill, acting as the coordinator of the Quantitative Biology honours and majors options in the Department of Biology, and the CS - Biology joint B.Sc. program.

Jackie’s research program is supported by NSERC, CIHR and the CFI.

Susi Kaitna

SUSI KAITNA

PD Research Associate

Genetic screens for elucidating the regulation of gamma-Tubulin interaction network.

Ph.D. Institute of Molecular Pathology
(Vienna, Austria)

Vincent Rouger

VINCENT ROUGER

Postdoc

Development of custom imaging and fluidics systems.

Ph.D. in Immunology at Centre d’Immunologie de Marseille Luminy (CIML) on biophotonics

Yohann Faivre

YOHANN FAIVRE

Postdoc

Kar9 mediated spindle alignmnent

PhD in Molecular and Cellular Biology at Claude Bernard University of Lyon (UCBL)

Eric Yen

ERIC YEN

Ph.D. Candidate

Developing new tools to investigate the structure-function relationships within protein complexes.

B.Sc. Quantitative Biology
(McGill University)

Supported by a fellowship from the CDMC CREATE program.

Kristy Shulist

KRISTY SHULIST

Ph.D. Candidate

Investigating the biological significance of multi-site phosphorylation in gamma-tubulin.

B.Sc. Honours Biology
(McMaster University)

Supported by an NSERC fellowship.

Allen Leary

ALLEN LEARY

Ph.D. Candidate

Applying physics and modeling techniques of biological systems.

B.Sc. , M.Sc. Physics
(McGill University and TRIUMF)

Supported by a fellowship from the CDMC CREATE program and a Trottier science accelerator fellowship.

Carlos G. Oliver

CARLOS G. OLIVER

Ph.D. Student

Developing computational and experimental tools to study the evolution of molecular systems.

B.Sc. Computer Science and Biology
(McGill University)

Supported by a fellowship from the CDMC CREATE program.

PUBLICATIONS

Ear PH, Kowarzyk J, Booth MJ, Abd-Rabbo D, Shulist K, Hall C, Vogel J and Michnick SW. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vitro Cdk1 Targeting Assay to Study the Regulation of the γ-Tubulin Complex. Methods Mol Biol, 1342:237-57 (2016)
Peter Swain, Keiran Stevenson, Allen Leary, Luis Montano-Gutierrez, Ivan Clark, Jackie Vogel, and Teuta Pilizota. Inferring time-derivatives including cell growth rates using Gaussian processes. Nature Comms (In Press)
Eric Yen, Aaron Tsay, Jerome Waldispuhl, Jackie Vogel*. Exploration of the dynamic properties of protein complexes predicted from spatially constrained protein-protein interaction networks. PLoS Computational Biology (2014).
Nazarova E, O'Toole E, Kaitna S, Francois P, Winey M and J Vogel. Distinct roles for anti-parallel microtubule pairing and overlap during early spindle assembly. Mol. Biol. Cell 15, 2013 vol. 24 no. 20 3238-3250 (2013)
Ear PH, Booth MJ, Abd-Rabbo D, Kowarzyk Moreno J, Hall C, Chen D, Vogel J* and SW Michnick* Dissection of Cdk1-cyclin Complexes in vivo *corresponding authors. PNAS vol. 110 no. 39 (2013)
Epp E, Nazarova E , Regan H, Douglas L, Konopka J, Vogel J, and M Whiteway. Clathrin- and Arp2/3-independent endocytosis in the fungal pathogen Candida albicans. mBio vol. 4 no. 5 (2013).
Hotz, M. Leisner C, Chen D, Manatschal C, Weglieter T, Ouellet J, Lindstrom D, Gottschling D, Vogel J and Y Barral. The spindle pole component Nud1 and the Mitotic Exit Network specify spindle pole body inheritance during yeast metaphase. Cell 148, 958-72 (2012).
Keck JM*, MH Jones*, CC L Wong, J Binkley, D Chen, S Jasperson, EP Holinger, T Xu, M Niepel, MP Rout, J Vogel, A Sidow, JR Yates III, and M Winey. A cell cycle phosphoproteome of the yeast centrosome. Science 332, 1557 (2011). *equal contribution
Rauch, A.* E Nazarova* and J Vogel. Analysis of microtubules in budding yeast. in: "Microtubules in vivo" Editors P. Tran and L. Cassemeris. Methods in Cell Biology 97, 277-306 (2010) *equal contribution
Mirzaei M., M. Pla-Roca, R. Safavieh, E. Nazarova, M. Safavieh, H. Li, J. Vogel and D. Juncker. Microfluidic perfusion system for culturing and imaging yeast cell microarrays and rapidly exchanging media. Lab Chip (2010) DOI: 10.1039/c004857g
Mui MZ, DE Roopchand, MS Gentry, RL Hallberg, J Vogel, PE Branton. Adenovirus protein E4orf4 induces premature APCCdc20 activation in Saccharomyces cerevisiae by a protein phosphatase 2A-dependent mechanism. J Virol. 84,4798-809 (2010).
Chen, D. and J Vogel. SAD kinase keeps centrosomes lonely. Nature Cell Biology 11,1047 (2009) invited review
Knockleby J and J Vogel. The COMA complex is required for Sli15/INCENP-mediated correction of defective kinetochore attachments Cell Cycle 8,2570 (2009) Comment: Amaro and Meraldi in the same issue of Cell Cycle
Logan ML, T Nguyen, N Szapiel, J Knockleby, H Por, M Zadworny, M Neszt, P Harrison, H Bussey, CA Mandato*, J Vogel* and G Lesage.* Genetic interaction network of the Saccharomyces cerevisiae type 1 phosphatase Glc7. BMC Genomics 15,336 (2008) *corresponding authors
Tarassov K,* V Messier,* CR Landry,* S Radinovic,* MM Serna Molina, I Shames, Y Malitskaya, J Vogel, H Bussey, SW Michnick. An in vivo map of the yeast protein interactome Science 320, 1465-70 (2008) *equal contribution
Cuschieri L, T Nguyen and J Vogel. Control at the cell center: the role of spindle poles in cytoskeletal organization and cell cycle regulation Cell Cycle 6, 2788-94 (2007) invited review
Ma L, J McQueen, L Cuschieri, J Vogel and V Measday. Spc24 and Stu2 promote spindle integrity when DNA replication is stalled. Mole. Biol. Cell 18, 2805-16 (2007)
Vogel, J. Scalable imaging solutions and systems integration. Apple Science White Paper Series (on-line publication July 20, 2007; www.apple.com/science/whitepaper)
Cuschieri L, M Miller and J Vogel. γ-Tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast. Mole. Biol. Cell 14, 4420-34 (2006). Cited as a study of special interest in Raynaud-Messina and Merdes, Curr. Opi. Cell Biol. (2007)
Pot I*, J Knockleby*, V Aneliunas, T. Nguyen, S Ah-Kye, G Liszt, M Snyder, P Hieter and J Vogel. Spindle checkpoint maintenance requires Ame1p and Okp1p. Cell Cycle 4, 1448-56 (2005) *equal contribution
Liakopoulos D*, J Kusch*, S Grava, J Vogel and Y Barral. Asymmetric loading of Kar9 onto spindle poles and microtubules ensures spindle alignment with the axis of the cell. Cell 112,561-74 (2003) *equal contribution
Vogel, JM, B Drapkin, J Oomen D Beach, K Bloom and M Snyder. Phosphorylation of γ-tubulin regulates microtubule organization in budding yeast. Dev. Cell 1,621-631 (2001). Reviewed in Usui and Schiebel, Mol. Cell (2001); Job et al. Curr. Opi. Cell Biol. (2003)
Vogel, JM and M Snyder. The carboxyl terminus of Tub4p is required for γ-tubulin function in budding yeast. J. Cell Sci. 113, 3871-82 (2000)
Vogel, J.M. and M. Snyder. γ-Tubulin of budding yeast. In: The Centrosome in Reproduction and Early Development. Curr. Topics in Devel. Biol. 49,75-104 (2000) Academic Press, San Diego, CA
RE Palazzo and JM Vogel. Isolation of centrosomes from Spisula solidissima oocytes. in: Mitosis and Meiosis. Methods In Cell Biology, 61,35-56. (1999) Academic Press, NY
Vogel, JM., T Stearns, CL Rieder, and RE Palazzo. Centrosomes isolated from Spisula oocytes contain rings and an unusual stoichiometric ratio of α / β tubulin. J. Cell Biol. 137,193-202 (1997)