VOGEL LAB

Interdisciplinary investigation of cellular division machinery
McGill University

ABOUT US

We use budding yeast to investigate the assembly and function of the mitotic spindle, and the control systems that govern asymmetric cell division.

Biological

Understanding spindle assembly and positioning through mutant analysis, high throughput genetic screening, protein-protein interaction assays, experimental evolution.

Computational

Applying modern computational methods to process and model complex biological data for hypothesis generation.

Physical

Elucidating the dynamics and forces that govern spindle positioning, assembly and function combining physical models and in-vivo fluorescence microscopy.

JOB OPPORTUNITIES

The lab is always interested in creative and motivated students and postdoctoral fellows to work in our collaborative interdisciplinary group.

The Vogel group is recruiting post-doctoral or graduate student applicants for spring or fall 2019. The successful applicant will join a inter-disciplinary research group focused on applying biophysical and computational methods to the study of spindle assembly.

Position 1: Biochemist, with a background in protein biochemistry, molecular biology and in vitro assays.
Project: Structure-function analysis of γ-tubulin and the γ-TuC, phosphoregulation of γ-tubulin
The applicant should have an interest in cell biology, biophysics and structural biology. Experience with microscopy, molecular biology methods, protein expression and purification, experience with Sf9 cell expression is particularly desirable.

Position 2: Cell and molecular biologist, background in biological or physical sciences.
Project: Control of spindle positioning during asymmetric cell division.
The applicant should have an interest in cell biology, biophysics and structural biology. Experience with microscopy, molecular biology and molecular genetics. Experience with MatLab/Python/FIJI scripting is desirable.

Undergraduate research opportunities for summer 2019: no open positions at this time.
Undergraduate students interested in experimental biophysics research projects are encouraged to apply for research courses for Fall 2019

RECENT PUBLICATIONS

Interphase microtubules safeguard mitotic progression by suppressing an aurora B-dependent arrest induced by DNA replication stress

Side chain electrostatic interactions and pH-dependent expansion of the intrinsically disordered, highly acidic carboxyl-terminus of γ-tubulin

Bipolar spindle formation is a fast, irreversible process requiring Kinesin-5 crosslinking and sliding modalities.

Interrogation of ɣ-tubulin alleles using high-resolution fitness measurements

Concerted millisecond timescale dynamics in the intrinsically disordered carboxyl terminus of ɣ-tubulin induced by mutation of a conserved tyrosine residue.

Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and pre-existing spindle poles

Inferring time-derivatives including cell growth rates using Gaussian processes

Exploration of the dynamic properties of protein complexes predicted from spatially constrained protein-protein interaction networks.

Distinct roles for antiparallel microtubule pairing and overlap during early spindle assembly.

PEOPLE

Jackie Vogel

JACKIE VOGEL

Group Leader

Jackie Vogel trained as a biochemist, focusing on mechanisms of centrosome maturation (Ph.D. University of Kansas, Ida Hyde and Candlin Scholar). Jackie studied the role of gamma-tubulin regulation in cell division at Yale University as a NIH and Leslie Warner post doctoral fellow (1996 - 2001). At McGill, Jackie has been a CIHR New Investigator (2004-2009) and most recently held the McGill-Canadian Pacific Biotechnology research chair (2009-2014). She is currently leading the Quantitative Biology Initiative at McGill, acting as the coordinator of the Quantitative Biology honours and majors options in the Department of Biology, and the CS - Biology joint B.Sc. program.

Jackie’s research program is supported by NSERC, CIHR and the CFI.

Maria Shadrina

MARIA SHADRINA

PD Research Associate

Computational chemistry/biology
protein structure-function analysis in silico

Ph.D Chemistry
Moscow State University (Russia)

Rachel Genthial

RACHEL GENTHIAL

Postdoc

Investigation of the formation and structure of the mitotic spindle using super-resolution / multimodal microscopy

PhD in Biophysics
Universite Grenoble-Alpes

Allen Leary

ALLEN LEARY

Ph.D. Candidate

Applying physics and modeling techniques of biological systems.

B.Sc. , M.Sc. Physics
(McGill University and TRIUMF)

Supported by a fellowship from the FRQNT and a Trottier science accelerator fellowship.

Shannon Sim

SHANNON SIM

M.Sc. Student

Biophysics; spindle forces, kinesin 5

B.Sc. Physics
McGill University

PUBLICATIONS

Laflamme G., Sim S., Leary A., Pascariu M., Vogel J and D'amours D. Interphase microtubules safeguard mitotic progression by suppressing an aurora B-dependent arrest induced by DNA replication stress. (2019) Cell Reports 26, 2875
Payliss B, Vogel J and Mittermaier A. Side chain electrostatic interactions and pH-dependent expansion of the intrinsically disordered, highly acidic carboxyl-terminus of γ-tubulin. (2019) Protein Science, in press DOI: 10.1002/pro.3618
Leary, A, E Nazarova, S Sim, K Shulist, P Francois and J Vogel. Bipolar spindle formation is a fast, irreversible process requiring Kinesin-5 crosslinking and sliding modalities. BioRXiv pre-print available doi: https://doi.org/10.1101/452821
Kristian Shulist*, Eric Yen*, Susanne Kaitna, Allen Leary, Alexandra Decterov, Debarun Gupta and Jackie Vogel. Interrogation of ɣ-tubulin alleles using high-resolution fitness measurements reveals a distinct cytoplasmic function in spindle alignment Scientific Reports 7:11398 (2017) *equal contribution
Jason Harris*, Maria Shadrina*, Carlos Oliver, Jackie Vogel^ and Anthony Mittermaier^ Concerted millisecond timescale dynamics in the intrinsically disordered carboxyl terminus of ɣ-tubulin induced by mutation of a conserved tyrosine residue. (2018) Protein Science DOI 10.1002/pro.3345. *equal contribution. ^corresponding authors.
Jette Lengefeld*, Eric Yen*, Xiuzhen Chen, Allen Leary, Jackie Vogel^ and Yves Barral1^ Spatial cues and not spindle pole maturation drive the asymmetry of astral microtubules between new and pre-existing spindle poles. MBOC (2017) DOI 10.1091/mbc.E16-10-0725 *equal contribution. ^corresponding authors.
Ear PH, Kowarzyk J, Booth MJ, Abd-Rabbo D, Shulist K, Hall C, Vogel J^ and Michnick SW^. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an In Vitro Cdk1 Targeting Assay to Study the Regulation of the γ-Tubulin Complex. Methods Mol Biol, 1342:237-57 (2016) ^corresponding authors
Peter Swain, Keiran Stevenson, Allen Leary, Luis Montano-Gutierrez, Ivan Clark, Jackie Vogel, and Teuta Pilizota. Inferring time-derivatives including cell growth rates using Gaussian processes. Nature Communications 7, 13766 (2016)
Eric Yen, Aaron Tsay, Jerome Waldispuhl^, Jackie Vogel^. Exploration of the dynamic properties of protein complexes predicted from spatially constrained protein-protein interaction networks. PLoS Computational Biology (2014). ^Corresponding authors
Nazarova E, O'Toole E, Kaitna S, Francois P, Winey M and J Vogel. Distinct roles for anti-parallel microtubule pairing and overlap during early spindle assembly. Mol. Biol. Cell 15, 2013 vol. 24 no. 20 3238-3250 (2013)
Ear PH, Booth MJ, Abd-Rabbo D, Kowarzyk Moreno J, Hall C, Chen D, Vogel J* and SW Michnick* Dissection of Cdk1-cyclin Complexes in vivo *corresponding authors. PNAS vol. 110 no. 39 (2013)
Epp E, Nazarova E , Regan H, Douglas L, Konopka J, Vogel J, and M Whiteway. Clathrin- and Arp2/3-independent endocytosis in the fungal pathogen Candida albicans. mBio vol. 4 no. 5 (2013).
Hotz, M. Leisner C, Chen D, Manatschal C, Weglieter T, Ouellet J, Lindstrom D, Gottschling D, Vogel J and Y Barral. The spindle pole component Nud1 and the Mitotic Exit Network specify spindle pole body inheritance during yeast metaphase. Cell 148, 958-72 (2012).
Keck JM*, MH Jones*, CC L Wong, J Binkley, D Chen, S Jasperson, EP Holinger, T Xu, M Niepel, MP Rout, J Vogel, A Sidow, JR Yates III, and M Winey. A cell cycle phosphoproteome of the yeast centrosome. Science 332, 1557 (2011). *equal contribution
Rauch, A.* E Nazarova* and J Vogel. Analysis of microtubules in budding yeast. in: "Microtubules in vivo" Editors P. Tran and L. Cassemeris. Methods in Cell Biology 97, 277-306 (2010) *equal contribution
Mirzaei M., M. Pla-Roca, R. Safavieh, E. Nazarova, M. Safavieh, H. Li, J. Vogel and D. Juncker. Microfluidic perfusion system for culturing and imaging yeast cell microarrays and rapidly exchanging media. Lab Chip (2010) DOI: 10.1039/c004857g
Mui MZ, DE Roopchand, MS Gentry, RL Hallberg, J Vogel, PE Branton. Adenovirus protein E4orf4 induces premature APCCdc20 activation in Saccharomyces cerevisiae by a protein phosphatase 2A-dependent mechanism. J Virol. 84,4798-809 (2010).
Chen, D. and J Vogel. SAD kinase keeps centrosomes lonely. Nature Cell Biology 11,1047 (2009) invited review
Knockleby J and J Vogel. The COMA complex is required for Sli15/INCENP-mediated correction of defective kinetochore attachments Cell Cycle 8,2570 (2009) Comment: Amaro and Meraldi in the same issue of Cell Cycle
Logan ML, T Nguyen, N Szapiel, J Knockleby, H Por, M Zadworny, M Neszt, P Harrison, H Bussey, CA Mandato*, J Vogel* and G Lesage.* Genetic interaction network of the Saccharomyces cerevisiae type 1 phosphatase Glc7. BMC Genomics 15,336 (2008) *corresponding authors
Tarassov K,* V Messier,* CR Landry,* S Radinovic,* MM Serna Molina, I Shames, Y Malitskaya, J Vogel, H Bussey, SW Michnick. An in vivo map of the yeast protein interactome Science 320, 1465-70 (2008) *equal contribution
Cuschieri L, T Nguyen and J Vogel. Control at the cell center: the role of spindle poles in cytoskeletal organization and cell cycle regulation Cell Cycle 6, 2788-94 (2007) invited review
Ma L, J McQueen, L Cuschieri, J Vogel and V Measday. Spc24 and Stu2 promote spindle integrity when DNA replication is stalled. Mole. Biol. Cell 18, 2805-16 (2007)
Vogel, J. Scalable imaging solutions and systems integration. Apple Science White Paper Series (on-line publication July 20, 2007; www.apple.com/science/whitepaper)
Cuschieri L, M Miller and J Vogel. γ-Tubulin is required for proper recruitment and assembly of Kar9-Bim1 complexes in budding yeast. Mole. Biol. Cell 14, 4420-34 (2006). Cited as a study of special interest in Raynaud-Messina and Merdes, Curr. Opi. Cell Biol. (2007)
Pot I*, J Knockleby*, V Aneliunas, T. Nguyen, S Ah-Kye, G Liszt, M Snyder, P Hieter and J Vogel. Spindle checkpoint maintenance requires Ame1p and Okp1p. Cell Cycle 4, 1448-56 (2005) *equal contribution
Liakopoulos D*, J Kusch*, S Grava, J Vogel and Y Barral. Asymmetric loading of Kar9 onto spindle poles and microtubules ensures spindle alignment with the axis of the cell. Cell 112,561-74 (2003) *equal contribution
Vogel, JM, B Drapkin, J Oomen D Beach, K Bloom and M Snyder. Phosphorylation of γ-tubulin regulates microtubule organization in budding yeast. Dev. Cell 1,621-631 (2001). Reviewed in Usui and Schiebel, Mol. Cell (2001); Job et al. Curr. Opi. Cell Biol. (2003)
Vogel, JM and M Snyder. The carboxyl terminus of Tub4p is required for γ-tubulin function in budding yeast. J. Cell Sci. 113, 3871-82 (2000)
Vogel, J.M. and M. Snyder. γ-Tubulin of budding yeast. In: The Centrosome in Reproduction and Early Development. Curr. Topics in Devel. Biol. 49,75-104 (2000) Academic Press, San Diego, CA
RE Palazzo and JM Vogel. Isolation of centrosomes from Spisula solidissima oocytes. in: Mitosis and Meiosis. Methods In Cell Biology, 61,35-56. (1999) Academic Press, NY
Vogel, JM., T Stearns, CL Rieder, and RE Palazzo. Centrosomes isolated from Spisula oocytes contain rings and an unusual stoichiometric ratio of α / β tubulin. J. Cell Biol. 137,193-202 (1997)